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Image Search Results
Journal: Frontiers in Oncology
Article Title: UHMK1 Is a Novel Marker for Personalized Prediction of Pancreatic Cancer Prognosis
doi: 10.3389/fonc.2022.834647
Figure Lengend Snippet: Knockdown of H19 or UHMK1 inhibits tumor growth in vivo. (A) siH19, siUHMK1, and a nonsense siRNA control (NC) were transfected into MIA-PaCa2 cells. After 24 h, 10 6 cells of each group were transplanted onto the CAM of each egg (n = 15 eggs/group) on developmental day 9 of the chick embryo. Tumor xenografts were resected on day 18 of chick development, and representative images are shown on the left. The individual xenograft volumes and the mean volumes of each group are presented on the right. (B) The expression of the Ki-67 proliferation marker was detected by immunohistochemistry in frozen xenograft sections. Representative images using 400× magnification are shown, and the scale bar represents 50 µm. The percentage of Ki-67-positive cells was quantified by counting the dark red-stained cells in 10 randomly chosen vision fields of each tissue by two independent examiners who were blinded by the conditions. (C) The expression of UHMK1 was detected and the percentage of the positive UHMK1 signal was quantified. ** P < 0.01.
Article Snippet: Primary antibodies included rabbit polyclonal antibodies against UHMK1 (PA550622, Invitrogen, Germany) and
Techniques: In Vivo, Transfection, Expressing, Marker, Immunohistochemistry, Staining